T7 RNA Polymerase (T7 RNAP) is a protein encoded by bacteriophage T7 DNA and expressed in recombinant E. coli. It is a DNA-dependent 5'→3' RNA polymerase that exhibits high specificity for recognizing the T7 promoter sequence. T7 RNA polymerase uses single-stranded or double-stranded DNA containing the T7 promoter sequence as a template and NTPs as substrates to synthesize RNA complementary to the template strand downstream of the promoter. This kit employs a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of T7 RNA Polymerase.
Key Features
• Target Enzyme: Specifically detects T7 RNA Polymerase, a DNA-dependent 5'→3' RNA polymerase with high T7 promoter specificity.
• Assay Principle: Double-antibody sandwich ELISA for accurate quantification.
• Quantitative Result: Provides precise quantification of T7 RNA Polymerase in test samples.
• Complete Workflow: Includes standard, pre-coated plate, enzyme-labeled reagent, TMB substrate, and stop solution (as per standard ELISA configuration).
